Books in the Advanced Methods series

The Advanced Methods series is intended for advanced undergraduates, postgraduates and established research scientists. Titles in the series are designed to cover current important areas of research in life sciences, and include both theoretical background and detailed protocols.

This title looks at the new microarray and microplate-based technologies which facilitate large-scale analysis of DNA sequence variants, mRNA molecules and proteins.

This volume should be invaluable for both new and established researchers in the field of human embryonic stem cells.

Covers the basic concepts and mechanisms of RNAi, transfection of cells with siRNAs, the design and validation of RNAi reagents, RNAi techniques in different organisms, large-scale RNAi screening, applications of RNAi in drug discovery, and potential uses of RNAi as a therapeutic agent. This work also highlights the pitfalls that can occur.

Provides the basic principles of applied real-time PCR. This work also provides a grounding for those who wish to develop further applications.

Molecular approaches to microbial ecology mean that in principle the entire range of species in a given sample can be analysed. This title provides a theoretical and methodological introduction to various key molecular techniques used in microbial ecology, focusing particularly on nucleic acid-based methodologies.

Cell Proliferation and Apoptosis provides a detailed and novel practical guide to cell proliferation and apoptosis detection methods.