Books in the Culture of Specialized Cells series

This book provides both general methods and safety considerations for the immortalization of cells and specific procedures for the immortalization of mammary, kidney, and thyroid epithelia, hepatocytes, fibroblasts, chondrocytes, lymphocytes, astrocytes, and human umbilical vein endothelium.

Step-by-step, practical guidance for the acquisition, manipulation, and use of cell sources for tissue engineering Tissue engineering is a multidisciplinary field incorporating the principles of biology, chemistry, engineering, and medicine to create biological substitutes of native tissues for scientific research or clinical use.

Presents techniques for initiation, propagation, and characterization of established cell lines. This book includes a listing of vendors for equipment and other cell culture products. It also discusses drug treatment, selection, differentiation, assays for malignant cells, risks, and applications.

DNA Transfer to Cultured Cells will describe the different updated methods and applications for transferring genetic material to cells in-vitro. Advances achieved in recent years to develop approaches for introducing DNA into cells have facilitated studies on the mechanism of expression of different important genes in the cells.

This guide provides a comprehensive synthesis of proven methods for the culture and experimental manipulation of haemopoietic cells. Covering a range of human and mouse cell types, and featuring step-by-step laboratory procedures, it gives detailed instructions for reagent preparation.