Books in the Methods in Enzymology series

Brings together a number of core protocols concentrating on protein, carefully written and edited by experts, including: pulse-chase analysis to measure protein degradation; labeling a protein with fluorophores using NHS ester derivitization; immunoaffinity purification of proteins; and proteolytic affinity tag cleavage.

Chemical and Synthetic Biology Approaches to Understand Cellular Functions - Part C, Volume 633, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial. This release includes sections on Next generation probes for molecular imaging in cells, Competitive binding assay for biotin and biotin derivatives, based on avidin and biotin-4-fluorescein, Converting avidin to bind ligands other than biotin, especially steroids, Chemoenzymatic Labeling Strategy, Engineered Siderophores, Small molecules to inhibit bacterial population behavior, NMR tube bioreactor, Small molecule controlled RAS activation system, Small molecule regulated Cas9, the Design and application of synthetic receptors, and much more. Contains the authority of authors who are leaders in their field Provides a comprehensive source on new methods and research in enzymology

Post-translational Modifications That Modulate Enzyme Activity, Volume 626 in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Updated chapters include Crosstalk between cellular metabolism and histone acetylation, Isolation of protein complexes and modifications that regulate transcriptional machinery, High-throughput phosphoproteome mapping through multiplexed mass spectrometry, Differentiation of D and L epimerization in proteins, Biochemical analysis of protein arginylation, Site-specific Determination of lysine acetylation stoichiometries on the proteome-scale, Genomic and biochemical analysis of RNA post-transcriptional modifications, Isolation and characterization of glycosylated (neuro)peptides, and more.Provides the authority and expertise of leading contributors from an international board of authorsPresents the latest release in the Methods in Enzymology seriesIncludes the latest information on Post-translational Modifications that Modulate Enzyme Activity

Enzymatic Polymerizations, Volume 627 in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field.Provides the authority and expertise of leading contributors from an international board of authorsPresents the latest release in the Methods in Enzymology seriesIncludes the latest information on Enzymatic Polymerizations

Enzyme Activity in Single Cells, Volume 628, the latest release in the Methods of Enzymology series, discusses groundbreaking cellular physiology research that is taking place in the biological sciences. Chapters in this new release cover Spatial and temporal resolution of caspase waves in single Xenopus eggs during apoptosis, Spatial and temporal organization of metabolic complexes in cells, Measuring cellular efflux and biomolecular delivery: synthetic approaches to imaging and engineering cells, Slide-based, single-cell enzyme assays, Single-cell assays using integrated continuous-flow microfluidics, High-throughput screening of single-cell lysates, Microfluidic capture of single cells for drug resistance assays, and much more. Provides the authority and expertise of leading contributors from an international board of authorsPresents the latest release in the Methods in Enzymology seriesIncludes the latest information on Enzyme Activity in Single Cells

Optochemical Biology, Volume 624, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters on a variety of topics, including Cell Lineage Tracing in Zebrafish Embryos with an Expanded Genetic Code, Optical Control of Tumor Induction in the Zebrafish, Optogenetic Control by Pulsed Illumination, Optimizing the Photocontrol of bZIP Coiled Coils with Azobenzene Crosslinkers: Role of the Crosslinking Site, Site-Directed RNA Editing in Vivo Can Be Triggered by the Light-Driven Assembly of an Artificial Riboprotein, In Situ Formation of an Azo Bridge on Proteins Controllable by Visible Light, and much more. Provides the authority and expertise of leading contributors from an international board of authorsPresents the latest release in the Methods in Enzymology seriesIncludes the latest information on optochemical biology

Biological NMR, Part B, the latest release in the Methods of Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of topics, including Protein methyl labeling, Membrane protein expression - yeast, Protein aromatic labeling, His-tag/Metal contamination, Bicelles, nanodiscs & micelles MP host, PTM - phosphorylation, PTM - lipidation, Screening platform for receptor-ligand discovery, Solution Spectroscopy, Large protein strategies, NUS data collection/analysis, F19 incl. hydration, ODNP - hydration, Reverse micelle - Hydration, Solid State Spectroscopy, SS NMR membrane proteins, SS NMR soluble/aggregate proteins, SS DNP - general, SS NMR nucleic acids, and much more. Authoritative contributorsProtocols for state-of-the-art advancesTimeliness

Single-Molecule Enzymology, Part B, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in single-molecule enzymology, and includes sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, and nanopore and tethered particle motion.Continues the legacy of this premier serial with quality chapters authored by leaders in the fieldCovers research methods in single-molecule enzymologyContains sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, and nanopore and tethered particle motion

Enzymology at the Membrane Interface, the latest volume in the Methods in Enzymology series, covers a subset of enzymes that work in the environment of the biological cell membrane. This field, called interfacial enzymology, involves a special series of experimental approaches for the isolation and study of these enzymes.Covers a subset of enzymes that work in the environment of the biological cell membraneOffers a series of experimental approaches for the isolation and study of enzymes

De Novo Enzyme Design, the newest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume includes the design of metal binding maquettes, insertion of non-natural cofactors, Cu metallopeptides, non-covalent interactions in peptide assemblies, peptide binding and bundling, heteronuclear metalloenzymes, florinated peptides, De Novo imaging agents, and protein-protein interaction. Continues the legacy of this premier serial with quality chapters on de novo enzyme designRepresents the newest volume in the Methods in Enzymology series, providing premier, quality chapters authored by leaders in the field Ideal reference for those interested in the study of enzyme design that looks at both structure and mechanism

cryoEM, a new volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods and new developments in recording images, the creation, evaluation and validation of 3D maps from the images, model building into maps and refinement of the resulting atomic structures, and applications of essentially single particle methods to helical structures and to sub-tomogram averaging.Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods that determine the structures of biological molecules, a vital step for understanding their functionContains the technical developments underpinning the advances of cryoEM and captures the exciting insights that have resulted