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The aim of this volume is to give recombinant DNA researchers current knowledge about heterologous gene expression in four commonly employed organisms: escherichia coli, bacillus subtilis, yeast and mammalian cells.
Part of the "Methods in Enzymology" series, this volume covers topics such as: total antioxidant activity; vitamin C; polyphenols and flavanoids; thiols; vitamin E and coenzyme Q10; and carotenoids and retinoids.
Provides information on prevention of oxidative stress; redox regulation of metabolism; toxicology; and, carcinogenesis.
Presents spectroscopic and physical methods for the determination of metal ion environments in metalloenzymes and metalloproteins. Major topics include spectroscopic methods for the determination of metal ion environments in metalloenzymes and metalloproteins.
Part of a series which contains material useful for researchers in various fields of life sciences. This title features spectroscopic detection of reaction intermediates; isotopic and kinetic detection of reaction intermediates; and, chemical trapping and inhibitor methods for detecting reaction intermediates.
Talks about fluorescence in biophysics; fluorescence as a tool for studying proteins; determination of ground-state dissociation constant by fluorescence spectroscopy; fluorescence spectroscopy of protein-protein and protein-nucleic acid interactions; fluorescence methods for studying equilibrium macromolecule-liquid interactions; and more.
This volume and its companion, Volume 214 of "Methods of Enzymology" seek to present a comprehensive, state-of-the-art compilation of the molecular and cellular methodology needed for pursuing research with carotenoids.
This first of two volumes provides up-to-date, methods-related information on ribonuclease functions, assays, and applications. Chapter topics include the identification of, characterization of, and assays for secreted ribonucleases; viral ribonucleases, artificial and engineered ribonucleases, and
Includes molecular, biological and biochemical methods for the study of cell surface heterotrimeric G proteins. Methods unique to signal transducing G proteins and general techniques applied to the study of G protein systems are covered. The companion issue, volume 238, covers G protein effectors.
This volume on phospholipases presents techniques for the assay, purification, and characterization of these enzymes, which are not only degradative enzymes of lipid metabolism but also key enzymes in the signal transduction pathway.
This volume, and its companions - "Methods of Enzymology" volumes 330 and 334 - cover contemporary knowledge concerning hyperthermophilic enzymes. Major topics in this volume include the enzymes of central metabolism, respiratory enzymes and biophysical and biochemical aspects of protein stability.
Part of the ""Methods in Enzymology" series, this volume describes the methods for detection and characterization of redox-active prosthetic groups, which arise from pre-existing amino acid side chains, in a range of enzyme systems.
Part of the series on Macromolecular Crystallography, this volume includes features such as: Crystal Growth; Phase Determination; Instrumentation and Data Collection; Model Building; and Refinement.
Presents methods related to the role of anaerobic dissimilatory cycles and sulfur metabolism. This volume deals with topics such as: sulfate transport, dissimilatory sulfate metabolism, dehydrogenases, hydrogenases from sulfate-reducing bacteria, electron carrier protein sulfate-reducing bacteria, and dissimilatory sulfur reduction.
Provides: a collation of the methods for the identification, preparation, and quantification of vitamins and coenzymes; details on physical, chemical, and biological properties of vitamins and coenzymes; chemical and biological syntheses of vitamins, coenzymes, and their analogs; and aspects of transport and metabolism of vitamins and coenzymes.
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