Books in the Methods in Molecular Biology series

This text provides a practical guide providing step-by-step protocol to design and develop vaccines. Volume 2: Vaccines for Veterinary Diseases includes vaccines for farm animals and fishes, vaccine vectors and production, vaccine delivery systems, vaccine bioinformatics, vaccine regulation and intellectual property.

This volume expands on statistical analysis of genomic data by discussing cross-cutting groundwork material, public data repositories, common applications, and representative tools for operating on genomic data.

This detailed volume explores the continuing techniques ofstudying RNA-protein complexes and interactions as research in these areasexpand. After an introductory chapter,the book continues with ways to purify RNA-protein complexes assembled in cellsor in isolated cellular extracts, methods for measuring various biochemicalactivities of RNA-interacting proteins or ribonucleoproteins, biochemicalmethods for measuring direct RNA-protein contact, as well as various new orinnovative methods pertinent to the subject. Written for the highly successful Methods in Molecular Biology series,chapters contain brief introductions to their respective topics, lists of thenecessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and up-to-date, RNA-ProteinComplexes and Interactions: Methods and Protocols provides a set of usefulprotocols, both basic and advanced, designed to inspire researchers workingwith RNA and RNA-interacting proteins.

Divided into several sections, the book delves into animal-pathogen interaction, microbe-microbe interaction, as well as plant-pathogen interaction.

The chapters compiled in this detailed collection outline a number of methods used to study plant mitochondria today, starting from the isolation of mitochondria to detailed analyses of RNA, protein and enzymatic activities.

This volume provides protocol references covering recent developments in the aptamer field. Within the last decade, aptamers have become more and more popular, and their sophisticated biophysical properties together with their ability to be easily modified and, thus, adapted to various regimens makes them a very promising class of compounds. Divided into three sections, the book covers selection, a series of analytical methods to assess biophysical properties of aptamer-target interactions, as well as various applications of aptamers. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Practical and easy to follow, Nucleic Acid Aptamers: Selection, Characterization, and Application provides a state-of-the-art summary of recent developments in the aptamer field and will be a helpful resource for scientists in the life sciences working with aptamers as tools to elucidate biological systems.

This volume focuses on procedures for the development and application of several research animal models and in vitro methods that allow researchers to gain insight into the underlying cellular, biochemical, and physiological mechanisms involved in traumatic and ischemic injury.

This detailed volume explores the use of Sertoli cells as models in male reproductive biology or as supporters for other cell types, and it answers the need for standardized protocols for their in vitro culture.

This volume looks at the latest methodologies used to study cellular metabolism with in silico approaches. The chapters in this book are divided into 3 parts: part I discusses tools and methods used for metabolic reconstructions and basic constraint-based metabolic modeling (CBMM); Part II explores protocols for the generation of experimental data for metabolic reconstruction and modeling, including transcriptomics, proteomics, and mutant generations; and Part III cover advanced techniques for quantitative modeling of cellular metabolism, including dynamic Flux Balance Analysis and multi-objective optimization. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, MetabolicNetwork Reconstruction and Modeling: Methods and Protocols is a valuable resource for qualified investigators studying cellular metabolism, and novice researchers who want to start working with CBMM.

This detailed volume explores the latest methods that can be used to probe mRNA decay pathways and identify mRNA-binding protein targets as well as miRNA targets.

This second edition expands on the first edition with new chapters describing methods for studyingcell movement, molecular components involved in chemotaxis, spatiotemporaldynamics of signaling components, and quantitative modeling, as well as severalupdated chapters from the first edition.

This volume provides a wide rangeof protocols used in studying the nuclear envelope, with special attention tothe experimental adjustments that may be required to successfully investigatethis complex organelle in cells from various organisms.

This volume includes a series ofprotocols focused on mitotic spindle assembly and function. The methods coveredin this book feature a broad range of techniques from basic microscopy to thestudy of spindle physiologies relevant to cancer. These methods can be appliedto diverse model systems that range from the cell-free Xenopus eggextract system to the moss Physcomitrella patens, in an effort todemonstrate the key contributions made by researchers using multiple modelorganisms. Chapters in The Mitotic Spindle: Methods and Protocols integrate cutting-edge technologies that have only become available due to thecross-disciplinary efforts, such as ATP analogue sensitive inhibition ofmitotic kinases. Written in the highly successful Methods in Molecular Biologyseries format, chapters include introductions to their respective topics,lists of the necessary materials and reagents, step-by-step, readilyreproducible laboratory protocols, and tips on troubleshooting and avoidingknown pitfalls.Thorough and informative, TheMitotic Spindle: Methods and Protocols, is a valuable resource forresearchers who are new to mitosis or are already experts in the field.

Chapters focus on of innate immune receptors, "atypical"inflammasomes, biochemical and novel bioluminescencetechniques for the measurement of IL-1b,bioluminescent probe, biochemical and microscopytechniques, techniques to measure caspase-1 activation, cellfree systems for the study of inflammasome function, and inflammasomeactivation.

This volume provides acollection of protocols and approaches for the creation of novel ligand bindingproteins, compiled and described by many of today's leaders in the field ofprotein engineering.

This volume details several important databases and data mining tools. Data Mining Techniques for the Life Sciences, Second Edition guides readers through archives of macromolecular three-dimensional structures, databases of protein-protein interactions, thermodynamics information on protein and mutant stability, ¿Kbdock¿ protein domain structure database, PDB_REDO databank, erroneous sequences, substitution matrices, tools to align RNA sequences, interesting procedures for kinase family/subfamily classifications, new tools to predict protein crystallizability, metabolomics data, drug-target interaction predictions, and a recipe for protein-sequence-based function prediction and its implementation in the latest version of the ANNOTATOR software suite. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Data Mining Techniques for the Life Sciences, Second Edition aims to ensure successful results in the further study of this vital field.

This volume containsprotocols specifically designed for studying programmed cell death, and alsodiscusses recent advances in techniques that span broader areas of biology thathave been recently used or that have potential to be incorporated into cell deathresearch.

With numerous methods available for the quantification ofnitric oxide (NO), this detailed book explores their advantages anddisadvantages in order to provide a foundation for further research in plantnitric oxide.

This volume provides a comprehensive collection of classical and cutting edge protocols and techniques to examine the normal development and physiological functions of the gastrointestinal system and to model the most common digestive diseases.

While additional chapters provide methods toculture human and mouse dendritic cells, protocols for the isolation ofdendritic cells, the isolation of dendritic cell progenitors from mouse, andthe purification of dendritic cells from human blood.

This detailed volume explores in silico methods forpharmaceutical toxicity by combiningthe theoretical advanced research with the practical application of the tools.Beginning with a section covering sophisticated models addressing the bindingto receptors, pharmacokinetics and adsorption, metabolism, distribution, andexcretion, the book continues with chapters delving into models for specifictoxicological and ecotoxicological endpoints, as well as broad views of themain initiatives and new perspectives which will very likely improve our way ofmodelling pharmaceuticals. Written for the highly successful Methods in Molecular Biology series,chapters include the kind of detailed implementation advice that is key forachieving successful research results.Authoritative and practical, In Silico Methods for Predicting DrugToxicity offers the advantage of incorporating data and knowledge fromdifferent fields, such as chemistry, biology, -omics, and pharmacology, toachieve goals in this vital area of research.

Thissecond edition expands upon the previous volume with new and updated chapters. Auditory and Vestibular Research: Methodsand Protocols, Second Edition guides readers through protocols on cellculture, tissue engineering, nanotechnology, high-throughput screening, andphysiology. Chapters on physiology covertechniques that include optical coherence tomography, patch clamping, andphotostimulation of caged neurotransmitters. Written in the highly successful Methods in Molecular Biology seriesformat, chapters include introductions to their respective topics, lists of thenecessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.Authoritativeand cutting-edge, Auditoryand Vestibular Research: Methods and Protocols, Second Edition aims to ensure successfulresults in the further study of this vital field.

Thisvolume covers multidisciplinary approaches on various aspects of ChikungunyaVirus (CHIKV) research that was brought together from leading laboratoriesacross the globe. Topics covered include techniques in clinical and diagnosticvirology, basic protocols in cell and virus culture, bioinformatics, andproteomics approaches in cellular response studies. Chikungunya Virus:Methods and Protocols also discusses methods in immunology and animal modelstudies, as well as different strategies of antivirals and vaccine developmentfor therapeutics against CHIKV infection. Written in the highly successful Methodsin Molecular Biology series format, chapters include introductions to theirrespective topics, lists of the necessary material and reagents, step-by-step,readily reproducible laboratory protocols, and tips on troubleshooting and avoidingknown pitfalls.Thorough and practical, Chikungunya Virus: Methods and Protocols is auseful and comprehensive resource for CHIKV researchers and anyone interestedin learning more about the Chikungunya Virus.

This volumepresents detailed laboratory protocols for invitro synthesis of mRNA with favorable properties, its introduction intocells by a variety of techniques, and the measurement of physiological andclinical consequences such as protein replacement and cancer immunotherapy.

Inaddition to updating important core techniques this third edition presents newchapters on assessing leukocyte involvement in angiogenesis, lymphatic cell andpericyte isolation techniques, spheroid and arterial ring based in vitroassays, and on pericyte involvement in angiogenesis.

This volume details a valuable collection of protocols andreviews, such as emerging experimental and theoretical approaches. Theseapproaches have resulted in a substantial improvement in the understanding ofchromosome architecture. Chromosome Architecture:Methods and Protocols guides readers through cutting-edge interdisciplinarymethods which allow for an understanding of architecture of chromosomes withexceptionally enhanced resolution, both in terms of space and time. Written in the highly successful Methods inMolecular Biology series format, chapters include introductions to theirrespective topics, lists of the necessary materials and reagents, step-by-step,readily reproducible laboratory protocols, and tips on troubleshooting andavoiding known pitfalls.Authoritativeand cutting-edge, ChromosomeArchitecture: Methods and Protocols aims to ensure successful results in thefurther study of this vital field.

This detailed volume encompasses chapters from leadingexperts in the area of membrane proteins who describe step-by-step protocols developed these last few years to improve the functionalproduction and stabilization of recombinant integral membrane proteins (IMPs).

The aim of this volume is to present current methodologies to predict and understand the pathogenesis of Type-1 Diabetes for clinical and non-clinical researchers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Practical and reliable, Type-1 Diabetes: Methods and Protocols will aid researchers in using these methods to advance their own studies.

Presenting topics from the basic application of molecular genetics to more complex gene expression analysis using different models of study, this detailed volume explores asthma through the lens of genetics, considered to play an essential role in the etiopathogenesis of the disease.