Books in the Methods in Signal Transduction Series series

Presents an analysis of various lipid signaling pathways. This book presents fresh experimental tools to investigate novel lipid mediators in signal transduction. It examines the role of lipid mediators in signal transduction and cell-to-cell communication.

Calcium Entry Channels in Non-Excitable Cells focuses on methods of investigating the structure and function of non-voltage gated calcium channels. Each chapter presents important discoveries in calcium entry pathways, specifically dealing with the molecular identification of store-operated calcium channels which were reviewed by earlier volumes in the Methods in Signal Transduction series. Crystallographic and pharmacological approaches to the study of calcium channels of epithelial cells are also discussed. Calcium ion is a messenger in most cell types. Whereas voltage gated calcium channels have been studied extensively, the non-voltage gated calcium entry channel genes have only been identified relatively recently. The book will fill this important niche.

This book aims to provide an up-to-date overview of the many signaling pathways regulating autophagy in response to different cellular needs. It explores the mechanisms and significance of cross-talk between autophagy and other cellular processes. Lastly, alterations in autophagy observed in aging and age-related pathologies is described.

T cells play a vital role mediating adaptive immunity, a specific acquired resistance to an infectious agent produced by the introduction of an antigen. There are a variety of T cell types with different functions. They are called T cells, because they are derived from the thymus gland. This volume discusses how T cells are regulated through the operation of signaling mechanisms. Topics covered include positive and negative selection, early events in T cell receptor engagement, and various T cell subsets.

Providing detailed methods for the study of transient receptor potential (TRP) channels and their role in a wide variety of cellular functions, this book includes expression and functional studies of TRP channels in heterologous systems as well as structural and functional studies of TRP channels. The author also explores various methods for detailed analysis of biophysical properties of TRP channels. In addition, the text covers high-throughput screening assays for TRP channels and offers novel and effective approaches to study endogenous TRP channel function in native systems.

This book describes methods used to investigate the function and distribution of lipids in membranes. Contributors present discoveries that have to do with lipid analysis and lipid interactions with other components of membranes, such as proteins, receptors, transporters and ion channels.

This book focuses on neuron signaling in the regulation of metabolism and body weight and associated methods. The book will cover recent progress on neuron signaling for body weight and metabolic regulation and the state-of-the-art methods. Readers will be exposed to latest research frontiers on neuron regulation of metabolism.

Presents an analysis of various lipid signaling pathways. This book presents fresh experimental tools to investigate novel lipid mediators in signal transduction. It examines the role of lipid mediators in signal transduction and cell-to-cell communication.