Books published by Cold Spring Harbor Laboratory Press,U.S.

Genome Science is a textbook and laboratory manual for advanced secondary and post-secondary education. It combines approachable narrative with extensively tested lab exercises that illustrate key concepts of genome biology in humans, invertebrates, and plants. Eighteen labs, organized into four chapters, engage students with both bioinformatics exercises and in vitro experiments. Each chapter also includes an extensive introduction that provides an historical and conceptual framework. This modular structure offers many options for enhancing existing courses, starting new courses, or supporting student research projects. The book is complete with advice for instructors, laboratory planning guidelines, recipes for solutions, and answers to student questions.

Written and edited by experts in the field, this collection from Cold Spring Harbor Perspectives in Biology focuses on the critical questions currently debated in the field of immune memory and vaccination. Topics include the plasticity of memory CD4 T cells, the formation of germinal centers by memory B cells, the contributions of natural killer cells to immune memory, and the influence of commensal microbiota on immune responses. Several contributors respond to each question, providing a variety of fresh, concise perspectives on each topic.

Color and learn! Welcome to your familyDLall the wonderful inhabitants of Planet Earth. We share something very special with every human being, animal, plant, and microscopic creature. What do we share with all living things? We are all GENE MACHINES! Discover all the books in the ENJOY YOUR CELLS series, each available in coloring book and full-color formats! Recommended for ages 7 and up.

Epilepsy affects millions worldwide and represents a variety of disorders characterized by seizures. This book examines the neurobiological bases of these conditions and current research into treatment strategies.

This collection reviews our understanding of the molecular mechanisms involved in establishing and maintaining cell polarity, as well as their relevance for human disorders. Contributors discuss how cells coordinate signaling pathways, membrane trafficking, and cytoskeletal remodeling to ensure proper spatial arrangement of cellular components. Processes such as transcytosis, exocyst-mediated vesicle trafficking, and endosomal sorting are covered in detail. Throughout, the focus is on epithelial cells and how polarity mechanisms facilitate the development of their apical and basolateral surfaces, tube formation, exchange of waste and nutrients, and the generation and maintenance of specialized structures at the cell surface.

The machinery that controls how DNA is packaged and genes are activated plays an important role in cancer. This book examines the functions of components of this machinery, how they can be dysregulated, and the prospects for targeting them therapeutically.

CRISPR-Cas-based techniques are revolutionizing molecular biology and genetics. The technology is based on a bacterial immune system that uses specific guide RNAs to target foreign DNA sequences for degradation. It has been adapted for genetic engineering and has a wide variety of applications in all fields of biology and the development of new therapeutic approaches.

Hepatitis B affects hundreds of millions of individuals worldwide and is a leading cause of liver cancer. This book examines our understanding of the biology of the Hepatitis B virus that causes the disease, the immune responses it elicits, and its role in liver cancer. It also discusses the related Hepatitis Delta virus and its effects.

Fungal infections affect millions of individuals worldwide. They a particular danger to immunocompromised individuals, such as those with HIV/AIDS, and invasive infections often have a mortality rate greater than 50%. This book examines our understanding of the biology of the major fungal pathogens, together with the host response, epidemiology of fungal diseases and current treatment strategies.

The successful isolation of specific cellular structures permits in-depth studies of their function and composition. This laboratory manual provides step-by-step protocols for the extraction of subcellular components from animal tissues, yeasts, plants, and cultured cells. Each chapter describes methods for isolating a particular organelle, vesicle, membrane, or macromolecular complex. Strategies for breaking cells without damaging the component of interest, enriching for that component based on its physical and biochemical characteristics, and monitoring and ensuring the success of the purification procedure are provided.

The successful isolation of specific cellular structures permits in-depth studies of their function and composition. This laboratory manual provides step-by-step protocols for the extraction of subcellular components from animal tissues, yeasts, plants, and cultured cells. Each chapter describes methods for isolating a particular organelle, vesicle, membrane, or macromolecular complex. Strategies for breaking cells without damaging the component of interest, enriching for that component based on its physical and biochemical characteristics, and monitoring and ensuring the success of the purification procedure are provided.

Retinal disorders are a leading cause of blindness. This book examines the molecular basis of these diseases, focusing on genetic approaches that are improving both our understanding of the disease process and their diagnosis, as well as advances in gene therapy that have been used to cure patients and provide a model for treatment of other conditions.

As universities increasingly spin off biotech companies and academics work more closely with these companies and the pharma industry, it is essential that they gain an understanding of intellectual property law and patenting. This book introduces the basic ideas and discusses how they apply to biomedical research and product development in the age of genomics.

Recent work has improved our understanding of the molecular and cellular bases of heart development and function. This book discusses these advances and the implications for diagnosis and treatment of heart disease, including stem cell therapy, tissue engineering, and transplantation.

As imaging technologies have revolutionized research in many areas of biology and medicine, neuroscientists have often pioneered the use of these new visualization techniques. This volume is an essential guide to discovering and implementing these techniques in the neuroscience lab.

One million cells in our bodies die every second--they commit suicide by a mechanism known as apoptosis. Apoptosis is essential for survival of the body as a whole and has critical roles in various developmental processes and the immune system. In Means To An End, Douglas Green provides a clear and comprehensive view of apoptosis and other cell death mechanisms. Taking a bottom-up approach, he starts with the enzymes that perform the execution process (a family of proteases termed caspases) and examines their cellular targets and the ways in which they are activated. He then looks at the molecular machinery that links signals that cause cell death to caspases, emphasizing the importance of the BCL-2 family of proteins and the role of cytochrome c released from mitochondria. The final stage of the process, phagocytic removal of dead or dying cells, is also covered. Green outlines the roles of apoptosis and death mechanisms such as necrosis in embryogenesis, neuronal selection, andthe development of self-tolerance in the immune system. In addition, he explains how cell death defends the body against cancer and traces the evolutionary origins of the apoptosis machinery back over a billion years. The book is thus of great use to all biologists interested in how cells function in the context of multicellular organisms and will appeal to everyone from undergraduates encountering the topic for the first time to researchers actively working in the field.