Books published by Springer US

This second edition volume expands on the previous edition with new information on the structural analysis of glycosaminoglycans (GAGs); chemical and enzymatic synthesis of GAGs; biophysical, biochemical, and computational analysis of GAG-protein interactions; molecular and genetic approaches to manipulating GAG cell biology; and molecular and genetic methods involving animal models and organoids. This volume also offers insight and guidance to non-glycoscience researchers who are performing advanced experiments on GAGs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. \Cutting-edge and authoritative, Glycosaminoglycans: Methods and Protocols, Second Edition is a valuable experimental manual for practicing researchersincluding graduate and post-doctoral fellows, and provides them with the latest methodologies and practical tips to overcoming barriers in understanding the chemistry and biology of GAGs.

This volume explores the latest techniques used to study the field of tissue engineered vascular grafts (TEVGs). The chapters in this book cover a wide array of topics such as deriving vascular cells from monocytes and induced pluripotent stem cells; engineering vascular grafts using various biomaterials and stem cells, stem cell-derived, or primary vascular cells; biomaterial modification by anticoagulation molecules; vascular bioengineering technologies such as 3D bioprinting; and fabrication of TEVGs with different geometry and multiphase structures. This book also features protocols for grafting and evaluation of vascular grafts in animal models, vascular imaging in animals, and the quantification of blood vessel permeability. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tipson troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Vascular Tissue Engineering: Methods and Protocols is a valuable resource for biomedical engineers, cell biologists, vascular surgeons, doctors, and nurses.

This volume presents a wide array of animal models that are used in the study of the individual and environmental factors involved in the development of addiction, the consequences of chronic drug exposure, and recently developed behavioral and biological procedures for the treatment of addictive disorders. The chapters in this book are organized into three sections that cover the aforementioned aspects in relation to different drugs of abuse and pathological gambling. The first section of the book focuses on the individual variables that affect vulnerability to addiction to nicotine, alcohol, cocaine, psychostimulants, and gambling. Section Two looks at the environmental variables that contribute to the vulnerability to addiction. Section Three explores the consequences of chronic drug consumption and new approaches to the treatment of addiction. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successfulresults in your laboratory.Cutting-edge and comprehensive, Methods for Preclinical Research in Addiction is a valuable resource that helps researchers and scientists understand the neurobiological mechanisms that underlie the influence of individual and environmental variables on the risk for addiction, and assists them in developing new behavioral and pharmacological strategies to prevent and treat addictive disorders.

How does homebanking work? How are board games developed? How reliable can wind energy get? How do we discover forged paintings? Do smart girls stay single? How dangerous can a bioterrorist get? In all these questions (and many others), mathematics plays a crucial role in the search for an answer.¿This book tells the story behind twenty of these questions. This is explicitly not a mathematics book, but a book about the crucial role that mathematics plays in devising the creative solutions the world needs. The questions are divided into three categories: mathematics in everyday life, mathematics for the workplace, and mathematics for tomorrow's society. The themes illustrate not only the incredibly broad applicability of mathematics in the world around us, but also the great diversity of useful mathematical techniques.