Books published by Springer US

This third edition handbook describes in detail the classical methods as well as extensions and novel approaches that were more recently introduced within this field. It consists of five parts: general recommendation techniques, special recommendation techniques, value and impact of recommender systems, human computer interaction, and applications. The first part presents the most popular and fundamental techniques currently used for building recommender  systems, such as collaborative filtering, semantic-based methods, recommender systems based on implicit feedback, neural networks and context-aware methods. The second part of this handbook introduces more advanced recommendation techniques, such as session-based recommender systems, adversarial machine learning for recommender systems, group recommendation techniques, reciprocal recommenders systems, natural language techniques for recommender systems and cross-domain approaches to recommender systems. The third part covers a wide perspective to the evaluation of recommender systems with papers on methods for evaluating recommender systems, their value and impact, the multi-stakeholder perspective of recommender systems, the analysis of the fairness, novelty and diversity in recommender systems. The fourth part contains a few chapters on the human computer dimension of recommender systems, with research on the role of explanation, the user personality and how to effectively support individual and group decision with recommender systems. The last part focusses on application in several important areas, such as, food, music, fashion and multimedia recommendation. This informative third edition handbook provides a comprehensive, yet concise and convenient reference source to recommender systems for researchers and advanced-level students focused on computer science and data science. Professionals working in data analytics that are using recommendation and personalization techniques will also find this handbook a useful tool. 

This volume covers a wide range of model systems from invertebrate to humans and in vitro and in vivo. Chapters detail methods on squid, aplysia, xenopus, mouse in vivo, ex vivo, rodent primary neurons, human-derived neurons, zebrafish, drosophila in vivo, primary neurons, c. elegans, and in vitro reconstitution assays.Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Axonal Transport: Methods and Protocols aims to further our understanding of the trafficking of cellular components in the nervous system leading to the advancement of basic knowledge that underlines the cell biology of the neuron.The chapters " Live imaging of RNA Transport and Translation in Xenopus Retinal Axons, Retrograde Axonal Transport of Neurotrophins in Basal Forebrain Cholinergic Neurons, Assessment of Mitochondrial Trafficking as A Surrogate of Fast Axonal Transport in Human Induced Pluripotent Stem Cell Derived Spinal Motor Neurons, Drosophila Primary Neuronal Cultures as a Useful Cellular Model to Study and Image Axonal Transport, and In vitro Reconstitution of Kinesin-Based, Axonal mRNA Transport" are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com

This detailed new edition provides a guide for implementing a selection of novel techniques utilizing the pioneering organism, Caenorhabditis elegans, a vital discovery engine for the rules of life, from molecules and genes, through cellular physiology, metabolism and aging, neurobiology and behavior, to individuality and interaction with the environment. Beginning with two chapters offering practical advice for setting up research programs with C. elegans, either for a new research group or for a short-term project such as a teaching laboratory, the volume continues with numerous hands-on methodologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, C. elegans: Methods and Applications, Third Edition offers the kind of clear guidance that will serve all researchers working with these vital and dynamic organisms.

This second edition is dedicated to new and updated methodological approaches designed to study galectin function. Chapters examine salient features of galectin functions. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Galectins: Methods and Protocols, Second Eidtion aims to be a useful practical guide to researches to help further their study in this field.

This second edition provides a comprehensive review of various gene silencing methodologies and applications. Chapters detail a historical overview of gene silencing mechanisms in plants, vectors, and strategies available for plant gene silencing, practical applications of gene silencing, bioinformatics tools, and other resources. In addition to these review chapters, this book includes methodology for virus-induced gene silencing (VIGS), understanding plant stress responses using VIGS, miRNA identification, DNA interference, host-induced gene silencing, artificial miRNAs for gene silencing, and high throughput RNAi. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Plant Gene Silencing: Methods and Protocols, Second Edition aims to further the understanding of functional relevance of target genes using gene silencing methods and technologies in commercial plant varieties.

This detailed volume is devoted to the recent development of quantitative experiments and computational methods driving new transforming growth factor beta (TGF-¿) and other cell signaling knowledge. Many chapters cover quantitative assays for TGF-¿ signaling studies, with others exploring the increasing role of both modeling and computational methods. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, TGF-Beta Signaling: Methods and Protocols serves as a vital resource for researchers seeking to move the TGF-¿ field into the quantitative realm.

This detailed book provides a broad collection of methodologies for natural killer (NK) cell research. Beginning with an assortment of methods for the isolation of NK cells and NK cell differentiation, the volume continues with methodologies for functional tests, such as cytotoxicity, viral infection, and metabolism assays, as well as clinical applications of NK cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Natural Killer (NK) Cells: Methods and Protocols serves as an ideal guide for researchers looking to contribute to the further development of basic and clinical NK cell research.

This fully updated book aims to facilitate the study of the nanochannels that connect plant cells, known as plasmodesmata, and to instigate new research that will further advance our knowledge of these structures. Beginning with the general structural composition and regulation of plasmodesmata as well as their role in plant development and disease, the volume continues with chapters exploring plasmodesmata architectures and distribution in cell interfaces, approaches to dissect plasmodesmata composition, protocols to quantify changes in plasmodesmata permeability using fluorescent tracers and mobile proteins, as well as a section with protocols that contribute to plasmodesmata research but fall outside the previous classifications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Plasmodesmata: Methods and Protocols, Second Edition serves as a vital guide for all plant scientists, both novice and expert, especially those studying the intricacies of cell-to-cell communication pathways.

This volume details current and new computational methodologies to study peptides. Chapters guide readers through antimicrobial peptides, foldability, amyloid sheet formation, membrane-active peptides, organized peptide assemblies, protein-peptide interfaces, prediction of peptide-MHC complexes, advanced free energy simulations for peptide binding, and methods for high throughput peptide or miniprotein design. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials, software, and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Computational Peptides Science: Methods and Protocols aims to provide concepts, methods, and guidelines to help both novices and experienced workers benefit from today's new opportunities and challenges.

This second edition volume expands on the previous edition by exploring the latest advancements in high throughput screening (HTS) in toxicity studies by using in vitro, ex vivo, and in vivo models. This volume also covers the application of artificial intelligence (AI) and data science to curate, manage, and use HTS data. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, High Throughput Screening Assays in Modern Toxicology, Second Edition is a valuable resource for scientists pursuing chemical toxicology research. This book will aid scientists and researchers in translating new HTS techniques into standardized chemical toxicology assessment tools that can refine, reduce, and replace animal testing.

This volume provides detailed methods and key approaches used to mechanistically study DNA damage, as well as the factors involved in the damage response. Chapters guide readers through proteomics and biophysical approaches, analyzing protein function, quantifying DNA replication dynamics and nucleic acid base damage, as well as biochemical reconstitution of key pathways involved in DNA repair. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, DNA Damage Responses: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field.

This detailed volume collects protocols from scientists who are actively engaged in developing or using protoplast technology. The book begins with chapters that focus on basic protoplast techniques and their utilities, such as protocols on protoplast isolation, transfection, and regeneration, as well as examples of how to use protoplasts for genome editing and gene function analysis in a number of major crop or model plant species. It continues by exploring protoplast automation, large scale functional genomics, and synthetic biology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Protoplast Technology: Methods and Protocols aims to inspire the new generation of researchers to further improve their protocols and apply this technology to accelerate the field of plant genomic study.

This volume provides state-of-the-art methods and protocols from researchers in the field of metabolism. Chapters guide readers through methodologies measuring different aspects of lipid metabolism, quantifying the amino acids, mitochondrial and intracellular by-products, detect enzyme and kinase activities, detecting a variety of metabolic pathways, and intracellular networks including nucleotide metabolism and the metabolic response to hypoxia and cytotoxic drugs. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Metabolic Reprogramming: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.

This detailed volume presents a diverse set of methodological approaches designed to improve our understanding of bacterial infections from a wide range of bacterial species. Beginning with biofilms and subcellular compartments, the book explores transcriptional analysis, methods for studying plasmid dynamics, and tools for phylogenetic analysis of bacterial genomes, as well as bacterial effector proteins interfering with host systems, host response analysis, and in vivo and in vitro infection models. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Bacterial Pathogenesis: Methods and Protocols, Second Edition is a vital resource for researchers in the area of infection biology, as well as but not limited to, those working in the fields of microbiology, immunology, structural biology, molecular biology, genetics, imaging, and computational study.

This volume presents the latest techniques to study several aspects of reproductive behavior in different species. The chapters in this book cover topics such as methods to investigate social behavior; sexual motivation and reward, both of which are fundamental for the initiation of sexual behavior; olfactory signaling; different characteristics and models of reproductive function; and parental behavior in both rats and rabbits. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and practical, Animal Models of Reproductive Behavior is a valuable resource for both experienced researchers and students who want to incorporate new techniques into their studies of reproductive behavior.

This volume provides a wide range of aspects related to mycoremediation, which can be applied for both basic and advanced multidisciplinary research. Chapters guide readers through screening of fungi, Polyaromatic Hydrocarbons (PAHs), textile dyes, pesticides, bioreactors, molecular methods, redictive Mycology and Proteomics approaches to select fungi, elucidating biological mechanisms, and fungal laccase enzyme-based biosensors for the detection of environmental contaminants. Authoritative and cutting-edge, Mycoremediation Protocols aims to be a practical guide on the functional properties of poorly known applications of fungi in order to find solutions for increasing environmental problems.

This book provides an up-to-date account of the most widespread methods used by specialists in the field of plant cytogenetics and the emerging field of cytogenomics that will likely soon be adapted by more labs. From the classical basic karyological approaches to the most recent genomics-informed and computational methods, the volume explores genome size and ploidy level estimation, chromosome fixation, preparation, and manipulation, banding and staining techniques, in situ hybridization, as well as numerous methods that integrate cytogenetics with bioinformatics and computational genomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Cytogenetics and Cytogenomics: Methods and Protocols serves as an ideal resource for plant scientists interested in molecular and evolutionary biology, breeding, systematics, and plant -omics in general.

This volume explores the latest experimental techniques in animal models of PTSD and humans affected by PTSD. The methods discussed in this book cover topics such as translational research; addressing sex differences; highlighting the state-of-the-art of biomarker discovery in the development and maintenance of PTSD; and looks at new promising agents to enhance fear extinction retention that may help millions of individuals that suffer from this debilitating disorder worldwide. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Authoritative and thorough, Translational Methods for PTSD Research is a valuable resource that will help researchers understand and learn more about this important disorder.

This book helps salespeople decode jargon and doublespeak commonly heard during the selling process and offers tips on how to move beyond ambiguous terminology and close the deal. Sorted into sales, marketing and management sections, Sales on the Go breaks each area down into five easy Q & A segments that highlight the most common and easily misunderstood phrases, comments, statements, and questions that salespeople hear every day. What to do when you encounter these phrases is spelled out in a simple to find, and easy to follow format which makes this book appealing to everyone with a sales job, whether yoüre just starting out or have years of experience.

This volume discusses the latest techniques used by researchers to measure cAMP activity at the cell population, whole cell, and subcellular level. The techniques covered in the book address questions related to cAMP compartmentalization, which look at relevant protein-protein interactions that increase the spatial and temporal resolution of cAMP signals detection, and that can help in the integration of the increasingly complex information that is becoming available in this field. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, cAMP Signaling: Methods and Protocols, Second Edition is a valuable resource for scientists and researchers who are interested in learning more about this important and developing field.  Chapter 7 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

This volume aims to introduce procedures related to measuring the process parameters involved in emerging food processing technologies, the approaches to measure the process efficiency, and basic guidelines for operating related systems. Chapters are divided into two parts, including nonthermal emerging food processing technologies and thermal emerging food processing technologies.Authoritative and cutting-edge,  Emerging Food Processing Technologies aims to provide comprehensive and updated state-of-art methodologies and models for food analysis.

This volume provides inflammasome instructions and tips to study different aspects of inflammasome biology. Chapters guide readers through how to assess canonical and non-canonical inflammasome activation, in vitro immune and non-immune cells, whole blood, biochemical assays to evaluate ASC oligomerization, gasdermin D cleavage, and mapping phosphorylated residues of NLRP3. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, The Inflammasome: Methods and Protocols aims to provide protocols that will serve as tools for current and aspiring researchers interested in studying inflammasomes.

This volume provides methods on different aspects and applications on plants, algae, photosynthetic bacteria, synthetic construct design, and multiplex cloning. Chapters cover multiple aspects of synthetic metabolic, photosynthetic systems, metabolic and signaling pathways, advanced engineering of metabolic networks, isolation of organelles and co-culture of microorganisms, and methods for the on command manipulation of the relative stability of proteins. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Plant Synthetic Biology: Methods and Protocols aims to be a useful resource for both researchers starting to explore novel experimental avenues as well as for experts willing to expand their portfolio of tools and strategies.

This second edition provides an overview of recent developments and approaches used by researchers to investigate the properties and functions of mammary epithelial and stem cells, which will contribute to understand the heterogeneity of the mammary gland and of breast cancer. Chapters detail processes used to characterize stem cells, single cell RNA sequencing, computational methods, sophisticated imaging techniques, and a variety of model systems, among others. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mammary Stem Cells: Methods and Protocols, Second Edition aims to make available protocols used to navigate the intricate behavior of mammary stem cells and to gain further knowledge to take us closer to the design of innovative strategies to prevent and treat breast cancer.

This volume presents an all-inclusive overview of molecular biological methods to study ABA signaling transport, and metabolism in several plant species. The chapters in this book cover topics such as biochemical and molecular biological characterization of ABA receptors; assay systems for ABA transporters; quantification and visualization of ABA levels; and comprehensive omics approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Abscisic Acid: Methods and Protocols is a valuable resource for scientists and researchers who are interested in learning more about this developing field.

This detailed volume explores techniques for researching brown adipose tissue (BAT) and the fascinating biology and therapeutic potential of thermogenic adipocytes. The content reflects the advancing technologies in genetics, imaging, and 'omics strategies that are allowing researchers to probe BAT biology at unprecedented depths and detail, yet it also presents classic physiology principles, which remain the core tenets of BAT biology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Brown Adipose Tissue: Methods and Protocols provides perspectives and detailed protocols for the benefit of both new BAT researchers looking for guidance as well as seasoned researchers who would like to expand their toolkits. Chapter 12 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

This volume explores the latest techniques used to study brain function and pathophysiology of major depressive disorder (MDD), and includes suggestions of new therapeutic approaches for the treatment of MDD. The chapters into this book are organized into five parts. Part One discusses advanced approaches to studying well-established pathophysiological mechanisms. Part Two details behavioral research methods for MDD. Part Three looks at the cellular and molecular research methods for major depression, and Part Four describes the latest developments in non-invasive neuroimaging. Part Five focuses on the pharmacological and non-pharmacological interactions, including antidepressant agents and their properties, such as sexual side effects and neuroimaging biomarkers. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.Cutting-edge and comprehensive, Translational Research Methods for Major Depressive Disorders is a valuable resource for researchers and scientists interested in learning more about this important and developing field.

This volume provides computational methods and reviews various aspects of computational studies of protein aggregation. Chapters discuss the relationship between protein misfolding and protein aggregation, methods of prediction of aggregation propensities of protein, peptides, protein structure, results of computer simulations of aggregation, and computational simulations focused on specific diseases such as Alzheimer's, Parkinson's, and preeclampsia. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Computer Simulations of Aggregation of Proteins and Peptides aims to ensure successful results in the further study of this vital field.

This volume explores detailed methods to investigate various aspects of biology related to cell polarity, or asymmetry within a cell. Molecular, cellular, and tissue-level regulation and function as well as diseases caused by impairment of cell polarity are explored by these methods. Beginning with advanced imaging and biochemical methods, the book continues with planar cell polarity (PCP) signaling in morphogenesis in diverse developmental contexts, apicobasal (AB) cell polarity in development and diseases, as well as directional cell migration and biomechanics in cell polarity. The collection includes the usage of a wide variety of model systems and an extensive array of techniques, including genetic, imaging, biochemical, and biomechanical. Written for the highly successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Cell Polarity Signaling: Methods and Protocols aims to enable researchers to delve into the stimulating field of cell polarity and contribute to our understanding of how coordinated control of protein stability, trafficking, membrane retention, post-translational modification, and dynamic organization leads to active regulation of cell polarity. Chapter 29 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

This detailed volume explores hepatic stellate cells, which have become a spotlight of liver cell biology due to their pleiotropic functions that extend well beyond extracellular matrix production to pivotal roles in hepatic homeostasis, immunity, and metabolism. The book features methods to isolate, evaluate, and manipulate this cell type in an effort to elucidate hepatic biology and establish prospects for treating disease. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Hepatic Stellate Cells: Methods and Protocols serves as an ideal aid to help researchers accelerate the outstanding science that has steadily unveiled the mysteries of stellate cell biology and their role in disease.