Books published by Springer-Verlag New York Inc.

This manual provides insights into the theory and practical aspects of several biotechnological and biochemical techniques for plants, protozoa, nematodes, insects and fishes, as well as human samples. The book also covers bioinformatics tools. The manual is an inclusive compilation, explaining techniques for microbial cultures, their diagnostics, DNA barcoding, microscopic techniques, blood analysis, parasite diagnostics through copro-antigens, enzyme analysis with enzyme kinetics, gene expression analysis, in-vivo protein visualization in live animals, geno-toxicity assays, quantification of micro RNAs and LncRNAs in tissue sections, the use of droplet PCR, and in-silico analysis. It provides step-by-step descriptions and details of each methodology, together with the final outcomes and inferences, in a simple and lucid manner, easily reproducible even for beginners. The broad range of techniques covered makes this volume extremely useful in understanding the principles of biotechniques, and simple applications for practical courses.

This book compiles the most comprehensive collection of protocols currently used in arbuscular mycorrhizal (AM) fungal research. This experience-based collection includes methods for isolation, cultivation, detection, and quantification of AM fungi, as well as the use of metagenomics for community studies and experimental procedures for functional genomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Arbuscular Mycorrhizal Fungi: Methods and Protocols serves as an ideal aid for researchers seeking to perform experiments that fill the gaps in our knowledge of the basic biology, functions, and ecology of AM fungi in the hope of deploying these powerful plant fungal symbiontsin agriculture more effectively.

This book compiles the most comprehensive collection of protocols currently used in arbuscular mycorrhizal (AM) fungal research. This experience-based collection includes methods for isolation, cultivation, detection, and quantification of AM fungi, as well as the use of metagenomics for community studies and experimental procedures for functional genomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Arbuscular Mycorrhizal Fungi: Methods and Protocols serves as an ideal aid for researchers seeking to perform experiments that fill the gaps in our knowledge of the basic biology, functions, and ecology of AM fungi in the hope of deploying these powerful plant fungal symbiontsin agriculture more effectively.

This book is a collection of classical as well as innovative methods used to investigate axon degeneration with a particular focus on addressing the common challenges encountered while performing these procedures. Particular attention is devoted to the study of axon loss in several model organisms, as each poses unique challenges and provides powerful advantages. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Axon Degeneration: Methods and Protocols is an ideal guide for facilitating the application and further development of these protocols, which will help the scientific community tackle important questions regarding axon degeneration.Chapters 2, 3, and 20 are availableOpen Access under a Creative Commons Attribution 4.0 International License via link.springer.com.

This fully updated volume presents a wide range of methods for synthesis, surface modification, characterization and application of nano-sized materials (nanoparticles) in the life science and medical fields, with a focus on drug delivery and diagnostics. Beginning with a section on the synthesis of nanoparticles and their applications, the book continues with detailed chapters on nanoparticle derivatization, bio-interface, and nanotoxicity, as well as nanoparticle characterization and advanced methods development. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Nanoparticles in Biology and Medicine: Methods and Protocols, Second Edition serves as an ideal guide for scientistsat all levels of expertise to a wide range of biomedical and pharmaceutical applications including functional protein studies, drug delivery, immunochemistry, imaging, and more.

The second editon of this popular ultrasound book expands the reader's understanding of the clinical applications of ocular ultrasound through a case study approach. With the addition of high-quality video segments of examination techniques not currently available in any other format, this edition appeals to a broader range of practitioners in the field by presenting the subject starting at the basic level and progressing to the advanced. The book is appealing to practitioners involved in ocular ultrasound, including ophthalmic technicians, ophthalmologists, optometrists, radiologists and emergency room physicians who, on occasion, are involved in the practice of ophthalmic ultrasound.

This thorough book collects methods and strategies to analyze proteomics data. It is intended to describe how data obtained by gel-based or gel-free proteomics approaches can be inspected, organized, and interpreted to extrapolate biological information. Organized into four sections, the volume explores strategies to analyze proteomics data obtained by gel-based approaches, different data analysis approaches for gel-free proteomics experiments, bioinformatic tools for the interpretation of proteomics data to obtain biological significant information, as well as methods to integrate proteomics data with other omics datasets including genomics, transcriptomics, metabolomics, and other types of data. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detailed implementation advice that will ensure high quality results in the lab. Authoritative and practical, Proteomics Data Analysis serves as an idealguide to introduce researchers, both experienced and novice, to new tools and approaches for data analysis to encourage the further study of proteomics.Chapter 16 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

For the last eighteen years, I have been teaching an introductory course in as­ trophysics. The course is intended for nonscience majors satisfying a general education requirement in natural science. It is a physics course with applications in astronomy. The only prerequisite is the high school mathematics required for ad­ mission to the university. For a number of years, I used an astronomy text, which I supplemented with lecture notes on physics. There are many good astronomy texts available, but this was not a satisfactory state of affairs, since the course is a physics course. The students needed a physics text that focused on astronomical applications. Over the last few years, I have developed a text which my students have been using in manuscript form in this course. This book is an outgrowth of that effort. The purpose of the book is to develop the physics that describes the behavior of matter here on the earth and use it to try to understand the things that are seen in the heavens. Following a brief discussion of the history of astronomy from the Greeks through the Copernican Revolution, we begin to develop the physics needed to understand three important problems at a level accessible to undergraduate nonscience majors: (1) the solar system, (2) the structure and evolution of stars, and (3) the early universe. All ofthese are related to the fundamental problem of how matter and energy behave in space and time.

Beginning with methods for the development and study of viruses, the book continues with current techniques and approaches used to develop new treatments from virus-derived vaccines to vectors and proteins, such as a range of models and methods designed to measure therapeutic efficacy of virus-derived biologics and therapeutic proteins.

Beginning with methods for the development and study of viruses, the book continues with current techniques and approaches used to develop new treatments from virus-derived vaccines to vectors and proteins, such as a range of models and methods designed to measure therapeutic efficacy of virus-derived biologics and therapeutic proteins.

This detailed second edition provides key updates on well-established methods as well as coverage of recently emerging sophisticated techniques and methods that are essential for an in-depth and state-of-the-art understanding of skeletal development and homeostasis and the pathological mechanisms that cause skeletal degeneration.

This detailed second edition provides key updates on well-established methods as well as coverage of recently emerging sophisticated techniques and methods that are essential for an in-depth and state-of-the-art understanding of skeletal development and homeostasis and the pathological mechanisms that cause skeletal degeneration.

This updated book explores a wide repertoire of tools and approaches that have been created, modified, and applied to the study of L. monocytogenes, forming the basis of our understanding of the bacterium today. Many of these key experimental techniques are gathered together herein. The volume presents aspects such as clinical disease and host-pathogen interactions, as well as the study of biofilms which present a significant challenge for control of the organism in the food processing environment. The topics covered in this edition also include sampling in order to isolate Listeria, methods for their identification and characterization, methods for gene manipulation, and methods for control of the organism. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Listeria monocytogenes: Methods and Protocols, Second Edition aims to contribute toward the harmonization of methods used to study this important bacterium, and to be of particular interest to Listeria research both in relation to food association and control as well as clinical microbiology.

Part I: Detection, Quantification, and Confirmation 1. Traditional Methods of Analysis for Listeria monocytogenes            Rui Magalh├úes, Cristina Mena, V├ónia Ferreira, Gon├ºalo Almeida, Joana Silva, and Paula Teixeira 2. MALDI-ToF MS: A Rapid Methodology for Identifying and Subtyping Listeria monocytogenes            Snehal R. Jadhav, Rohan M. Shah, and Enzo A. Palombo 3. Sample Preparation for qPCR Detection of Listeria from Food            Patrick Mester, Anna Kristina Witte, and Peter Rossmanith 4. qPCR Validation on the Basis of the Listeria monocytogenes prfA Assay            Anna Kristina Witte, Patrick Mester, and Peter Rossmanith Part II: Subtyping Approaches 5. Serotype Assignment by Sero-Agglutination, ELISA, and PCR            Lisa Gorski 6. Pulsed-Field Gel Electrophoresis (PFGE) Analysis of Listeria monocytogenes            Karen Hunt and Kieran Jordan 7. Multi-Locus Sequence Typing (MLST) and Whole Genome Sequencing (WGS) of Listeria monocytogenes and Listeria innocua            Beatrix Stessl, Martin Wagner, and Werner Ruppitsch Part III: Genotypic and Phenotypic Characterization 8. High-Throughput Characterization of Listeria monocytogenes Using the OmniLog Phenotypic Microarray            Laura Luque-Sastre, Kieran Jordan, S├⌐amus Fanning, and Edward M. Fox 9. High-Throughput Screening of Biofilm Formation of Listeria monocytogenes on Stainless Steel Coupons Using a 96-Well Plate Format            Jessica A. Gray, P. Scott Chandry, John P. Bowman, and Edward M. Fox 10. Confocal Laser Microscopy Analysis of Listeria monocytogenes Biofilms and Spatially Organized Communities            Maud Darsonval, Marina Gr├⌐goire, Julien Deschamps, and Romain Briandet 11. Extraction and Preparation of Listeria monocytogenes Subproteomes for Mass Spectrometry Analysis            Tiago Santos and Michel H├⌐braud Part IV: Strain Manipulation 12. Extraction and Analysis of Plasmid DNA from Listeria monocytogenes            Aidan Casey and Olivia McAuliffe 13. Generation of Nonpolar Deletion Mutants in Listeria monocytogenes Using the "SOEing" Method            Kathrin Rychli, Eva Harter, Caitriona M. Guinane, Karen Daly, Colin Hill, and Paul D. Cotter 14. Mutant Construction and Integration Vector-Mediated Genetic Complementation in Listeria monocytogenes            Cameron Parsons, Reha Azizoglu, Driss Elhanafi, and Sophia Kathariou 

This Open Access volume provides readers with an open access protocol collection and wide-ranging recommendations for preclinical renal MRI used in translational research.

This detailed second edition presents a wide variety of marine invertebrate model systems, from cephalochordata to holothurians, along with novel experimental protocols for taking advantage of their unique properties.

This detailed second edition presents a wide variety of marine invertebrate model systems, from cephalochordata to holothurians, along with novel experimental protocols for taking advantage of their unique properties.

Preface...Table of Contents...Contributing Authors...1.Ex Vivo Culture for Preimplantation Mouse Embryo to Analyse PluripotencyKatia Boutourlinsky, Nicolas All├¿gre, and Claire Chazaud2.Microfabricated Device for High Resolution Imaging of Preimplantation EmbryosSandrine Vandormael-Pournin, Emmanuel Frachon, Samy Gobaa, and Michel Cohen-Tannoudji3. In Vitro Culture of Mouse Blastocyst to the Egg Cylinder Stage via Mural Trophectoderm ExcisionHatice O. Ozguldez and Ivan Bedzhov4. Spatially Organized Differentiation of Mouse Pluripotent Stem Cells on Micropatterned SurfacesSophie M. Morgani and Anna-Katerina Hadjantonakis 5. Mouse Primordial Germ Cells: In Vitro Culture and Conversion to Pluripotent Stem Cell LinesMalgorzata Borkowska and Harry G. Leitch6. Generation of Primordial Germ Cell-Like Cells in Small and Large ScalesWolfram H. Gruhn and Ufuk G├╝nesdogan7. Genome-Scale CRISPR Screening for Regulators of Cell Fate TransitionsValentina Carlini, Kristjan H. Gretarsson, and Jamie A. Hackett8. Somatic Reprogramming by Nuclear Transfer Vincent Brochard and Nathalie Beaujean9. Targeted Transgenic Mice using CRISPR/Cas9 TechnologyFatima El Marjou, Colin Jouhanneau, and Denis Krndija10. Whole-Mount Immunofluorescence Staining of Early Mouse EmbryosFrederick C. K. Wong  11. Investigating the Inner Cell Mass of the Mouse Blastocyst by Combined Immunofluorescence Staining and RNA Fluorescent In Situ HybridizationMaud Borensztein 12. Mapping of Chromosome Territories by 3D-Chromosome Painting during Early Mouse DevelopmentKatia Ancelin, Yusuke Miyanari, Olivier Leroy, Maria-Elena Torres-Padilla, and Edith Heard13. Deciphering the Early Mouse Embryo Transcriptome by Low-Input RNA-seqRaquel P├⌐rez-Palacios, Patricia Fauque, Aur├⌐lie Teissandier, and D├⌐borah Bourc''his14. Studying DNA Methylation Genome-Wide by Bisulfite Sequencing from Low Amounts of DNA in MammalsAmbre Bender, Hala Al Adhami, Thomas Dahlet, and Michael Weber15. Profiling DNA Methylation Genome-Wide in Single CellsAntonio Galvao and Gavin Kelsey 16. Tracking Histone Modifications in Embryos and Low Input Samples using Ultrasensitive STAR ChIP-seqBingjie Zhang, Xu Peng, Feng Xu, and Wei Xie17. Chromatin Profiling in Mouse Embryonic Germ Cells by CUT&RUNSrinivasa Abishek Prakash and Joan Barau 18. DamID to Map Genome-Protein Interactions in Pre-Implantation Mouse EmbryosMrinmoy Pal, Kim de Luca, Jop Kind, and Maria-Elena Torres-Padilla19. Understanding Chromosome Structure during Early Mouse Development by Single-Cell Hi-C AnalysisNo├⌐mie Ranisavljevic, Maud Borensztein, and Katia Ancelin 20. Bioinformatic Analysis of Single Cell Hi-C Data from Early Mouse EmbryoSamuel Collombet, Yuvia A. P├⌐rez-Rico, Katia Ancelin, Nicolas Servant, and Edith Heard Subject Index List...