Books in the THE BASICS Garland Science series

This book describes the basic requirements for establishing and maintaining cell cultures both in the laboratory and in large-scale operations. Minimal background knowledge of the subject is assumed and therefore it will be a readable

Plant cell culture is an essential methodology in plant sciences, with numerous variant techniques depending on the cell type and organism. This book provides the reader with a concise overview of these techniques.

The second edition of Protein Purification expands on the topics covered previously. It discusses the structure and properties of water and emphasizes properties which can be exploited to aid purification. It features an updated suppliers list and chapters feature an extension of protocols that are discussed.

Provides an introduction to the main methods of measuring gene expression.

Including a chapter on Real-Time PCR, prokaryotic applications, and details in the complex mutagenesis sections, this text aims to integrate information on PCR applications in genomics and proteomics.

A basic guide to working with and cloning DNA, starting with laboratory supplies, reagents, stock solutions, buffers, and record keeping. It then goes on to give advice on choosing the correct enzymes and vectors in order to achieve an appropriate product and finally discusses how to identify the product.