Subcellular Fractionation: A Laboratory Manual

The successful isolation of specific cellular structures permits in-depth studies of their function and composition. This laboratory manual provides step-by-step protocols for the extraction of subcellular components from animal tissues, yeasts, plants, and cultured cells. Each chapter describes methods for isolating a particular organelle, vesicle, membrane, or macromolecular complex. Strategies for breaking cells without damaging the component of interest, enriching for that component based on its physical and biochemical characteristics, and monitoring and ensuring the success of the purification procedure are provided.